11 Mushroom Substrate Recipes (DIY Options That Work!) - [2024] (2024)

Mushrooms are a great addition to any diet, and they can be grown in your own home with the right substrate recipes. There are many different DIY substrates you can use, but some of them work much better than others.

Here are eleven of the best mushroom substrate recipes that will help you get started growing your own mushrooms at home!

Note: If you click some of the links or pictures in this article we may earn a small commission at no additional cost to you.

Commonly Used Substrates for Growing Mushrooms

Due to the fact that sawdust and pellets are useful for growing any kind of mushroom, these are the most popular substrates. In addition, you’re likely to find the following:

  • Fanaticus mixture, best for cubensis
  • Coffee Grounds, great for shiitake and oysters
  • Straw, best for oysters
  • Rye grain, which grows fast so it’s good for making spawns of other types
  • Manure, great for mushrooms that naturally grow in it like buttons
  • Logs, best for all fungus that grows on dying trees
  • Coco coir and vermiculite, which can serve as a substitute for manure

Coffee Substrate Recipe for Oyster Mushrooms

Sterilize regular spent coffee grounds and add them to a clear plastic bag. You may want to add around a tablespoon of gypsum to prevent the material from sticking to itself.

Adding at least some rye grains to the mixture will help to provide extra nutrition, which is important when growing oyster mushrooms.

Hardwood Sawdust Recipe for Shiitake Mushrooms

Around a single pound of hardwood sawdust mixed with a pound or more of soy bean hull can produce big shiitake flushes. You’ll want to add just under 3 pints of water to keep it from getting too dry.

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PF-TEK Recipe for Cubensis Mushrooms

Sterilize the following ingredients in a pressure cooker to make a good PF-TEK mix:

  • 60 mL water
  • ½ cup Vermiculite
  • ⅙ cup brown rice flour

These should be processed in a pressure cooker for around 45 minutes to ensure cleanliness.

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Straw Recipe for Blue Ostreatus

Straw, air and an appropriately sized fruiting container is all you’ll need to grow these large meaty mushrooms. They’re going to require more air than normal, though, which is why they’re a favorite of those who prefer to do all of their growing in the great outdoors.

Some people add at least some portion of coffee grounds in order to give their mushrooms some extra nutrients.

We recommend the chopped straw below. Click the image to check the current price on Amazon.

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Rye Grain Recipe for Spawn

Soak rye grains in water and add a cup of used coffee grounds. Add a tablespoon of calcium sulfate dihydrate to keep it from clumping together.

This can be used to grow any kind of mushroom spawn, though it’ll have to be transplanted to its preferred type of substrate once it’s begun to fruit. Most mushroom species don’t produce spawn that’s edible in any quantity.

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Manure Recipe for Button Mushrooms

Mushroom growers tend to get creative when they’re mixing together manure, but you’ll usually want to base your substrate around two parts manure to one part coco coir. Add water to get it to the right field capacity, but don’t let it start to pool or drip because button mushrooms don’t like that.

Fungus growing expert Oliver Carlin recommends using a pressure cooker for around 2½ hours at 15PSI to sterilize it. This is especially important when dealing with natural sun dried manure, which understandably has a large amount of bacteria in it.

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Log Recipe for Shiitake Mushrooms

Rather than a recipe per se, you’re going to need a hardwood log that’s around 3-4 ft in length. Most mushroom growers recommend making sure that it’s at least 5 inches in diameter, though you don’t want to exceed maybe half a foot in width.

Drill inch-deep holes every four inches in a row. Stagger these in a diamond-shaped formation for best results and make sure to clear them out before you start to introduce shiitake spawn into them.

Coco Coir Recipe for Psilocybe

In general, you’ll want to use twice as much sun-dried horse manure for every part of coco coir you have. Therefore, if you have 8 lbs. of coco coir you’ll want to add an additional 16 lbs. of manure to it for growing mushrooms.

Most people won’t need to put together anything near this size, but it’s possible to do so. A good rule of thumb is to keep the entire mixture together around 10 lbs. or so to make the mixture manageable.

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Used Cardboard Recipe for Pink Ostreatus

Cardboard should be carefully soaked in boiling water, so you’ll probably have to rip it apart somewhat before you start to use it to grow mushrooms. Bring a pot with cardboard in it to a boil and then let it cool down naturally.

Preferably, you’ll want to use cardboard that hasn’t been bleached or dyed. Traditional packing containers, as well as the plain brown paper they come wrapped in, work best for this kind of mixture.

Some people have reported that even used pizza boxes work. You may want to remove any food scraps if you go this route.

Grain Recipe for Spawn Growth

Barley or even popcorn granules can be used in place of rye for growing mushroom spawn of nearly any species. Rinse your grains and put them into a pressure cooker with enough water for them to absorb.

Cook them for a half hour at 15PSI and then let them drain once it’s safe to open the cooker. You’ll want to pat the corns dry and place them into mason jars for later sprouting.

A few mushroom experts have suggested using poly fill to take up any extra space in the jars.

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How to Mix Substrates to Create Your Own Recipe

While you’ll want to be creative, you need to be mindful of what different substrates are like. Grains, for instance, can take the place of other grains.

Dead wood like logs can be interchanged with cardboard because they’re from the same family of materials. Don’t be afraid to experiment, but keep this in mind because you could get undesirable results if you try to grow mushrooms on the wrong type of substrate.

Add water to your mixture in small intervals. Don’t ever let it start to pool together regardless of how dry your substrate mix might be.

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John Stephens

Hi, I’m John Stephens, chief editor and writer for Totalgardener.com. I’ve been gardening and raising animals for over 15 years starting with a small backyard plot in Northern Virginia where I grew corn, potatoes, squash, and using a high mulch technique called the Ruth Stout Method. I also raised ducks and small mammals for meat and eggs in a movable pen similar to the ones used by Joel Salatin. I later moved to Colorado where I experimented with growing greens using aquaponics inside. I eventually added a microgreens setup and home sprouting operation. I’m excited to share everything I’ve learned plus more from the other local gardening and animal raising experts I know.

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11 Mushroom Substrate Recipes (DIY Options That Work!) - [2024] (2024)

FAQs

How do you make a homemade substrate? ›

Recipes usually call for two parts manure with one part coco coir. Water then needs to be added to reach field capacity. That is basically the amount of water the substrate can hold without extra water pooling at the bottom.

What can I use as a substitute for mushroom substrate? ›

... Alternatives for mushroom cultivation has been reported with varying success including wheat straw, cottonseed straw, cereal straw, corncob, sugar cane straw and sawdust.

What is the bulk substrate for cubensis? ›

Manure/Compost

Manure is the aged, dried excrement of horses, cows, elephants, etc. It is one of the most effective bulk substrates for dung loving species like psilocybe cubensis, panaeolus cyanescens and agaricus bisporus (Portobello). It is usually cheap or free if it can be located.

How do you make a good mushroom substrate? ›

Hardwoods such as oak, beech and maple make for a great substrate for many types of mushrooms, especially when combined with a bran supplement. Soy hulls can be mixed with hardwood sawdust at different ratios to produce extremely effective and high yielding substrates, particularly for growing oysters.

What is the easiest mushroom substrate? ›

Pretty much every mushroom growing resource I could find says that oyster mushrooms are the easiest variety for first time-growers, as they grow fast and can easily thrive in substrates made of things like coffee grounds and straw, making them relatively low maintenance.

Can I use wood chips for mushroom substrate? ›

For mushrooms though, the substrate provide a home and a source of nutrients for the mycelium. It is important to acknowledge the different types of mulch because the type of mulch you use is dependent on what mushroom you want to grow. For most mushrooms this usually mean wood chips or straw (not hay).

What is the best substrate for a Monotub? ›

Best is manure that has been composted for at least 6 months. At this point you can either pasteurize your bulk substrate by cooking it in an oven or steaming it. You'll need to mix the two ingredients together and hydrate the mixture to 'field capacity.

What is the best size Monotub cubensis? ›

A monotub should be 54 quarts, more or less. That's the size of monotub we always use, and we highly recommend that you do the same, whether you're planning on ordering our All-in-One kit or not!

What is the best substrate for mushroom spawn? ›

Many different types of grains can be used for spawn – including wheat, rye, brown rice, white rice, wild bird seed and even popcorn. Without a doubt, however, the most common grain used for mushroom cultivation is Rye.

Which is the most commonly used substrate for mushroom production? ›

Agricultural wastes are the good source for the cultivation of mushrooms. Some of them are most commonly used such as wheat straw, paddy straw, rice straw, rice bran, molasses, coffee straw, banana leaves, tea leaves, cotton straw, saw dust etc.

Why add gypsum to mushroom substrate? ›

Gypsum has been found to shorten the time it takes for the mycelium to grow throughout the substrate, according to researchers at the University of Putra Malaysia. That means it's quicker for the spores to grow throughout the growing block and start to grow mushrooms.

How do you make Masters mix substrate? ›

The masters mix is pretty straight forward: “Combine 1 part hardwood sawdust with 1 part soy hulls, hydrate to 60%, and sterilize at 15 PSI for 2.5 hours.” It is simply A 50/50 mix of soy bean hulls and hardwood sawdust hydrated to perfection!

Can you use just coco coir for mushroom substrate? ›

And before all to start, yes, you can use coco coir to grow mushrooms. It is one of the best to choose from, and let's dig deeper.

What material is best for substrate? ›

Rigid substrate – For strength and a strong dielectric

Some of the most renowned rigid substrates are ceramic-based. Two common examples are alumina (Al2O3) and aluminum nitrate. The latter offers high thermal conductivity (i.e., high thermal strength with low expansion).

How do you make a sterile substrate? ›

To sterilize a substrate, you need temperatures higher than 250°F (121°C) for at least 2 hours. Boiling water or steam on its own is not hot enough to sterilize a substrate as the boiling temperature of water at sea level is 212°F (100°C), and at higher altitudes, it's even lower.

Can you use oatmeal for substrate? ›

Answers. You can use oatmeal (not instant,) corn meal, masa, or wheat bran. We recommend using organic grain if you can to avoid all pesticides.

How do you sterilize your own substrate? ›

Anything that raises the temperature of your substrate above 120°C for a long amount of time can sterilise it. This involves using an oven or autoclave to sterilise your substrate.

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